Xiaowei Zhuang (Harvard/HHMI) Part 1: Super-Resolution Fluorescence Microscopy

https://www.ibiology.org/biophysics/super-resolution/

Zhuang begins her lecture by explaining that the resolution of traditional light microscopy is about 200 nm due to the diffraction of light. This diffraction limit has long hampered the ability of scientists to visualize individual proteins and sub-cellular structures. The recent development of sub-diffraction limit, or super resolution, microscopy techniques, such as STORM, allows scientists to obtain beautiful images of individual labeled proteins in live cells. In Part 2 of her talk, Zhuang gives two examples of how her lab has used STORM; first to study the chromosome organization of E. coli and second, to determine the molecular architecture of a synapse.

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Comment (55)

  1. Many thanks for this great presentation, very clearly explained.

     But now we just need to win the lottery to get a storm instrument to play with.

  2. Thanks for breaking this down so simply. It is amazing just how detailed the images are and humbling to realize that each of the cells imaged are so vastly complex and compact. Exciting to imagine how much further this can be improved. Thanks again I really felt like I learned a lot.

  3. This video is exactly what I was looking for. I had trouble to understand super resolution microscopy, and now I think I understood the core concept of it. Thank you so much!!

  4. I am only 6 minutes in and already I approve of your presentation. I am a layperson who studies the sciences purely for self enlightenment. Unfortunately I was unable to pursue my passion for a science career in my youth due to financial issues. Nicely done and very informative.

  5. It will be good for cryonicists and I have the same disease than Howard Hughes almost. But I hope this tool will widely be used by scientists in the future to see if we can save our own lives. like in anti aging. all around the world. in germany, in france, in china, in canada, in usa etc..

  6. for cryonicists we will need more than that, more than 10 NM resolution, and not only cells outsides the bodies, but inside the bodies like in a MRI, while the patient is living or not, like frozen or where there are movement. This tool was implemented in 2006 I think or so, and perfected in 2018 or 2016. Let's put another 100 years and we will be able to see in a resolution of less than 1 nanometers, everywhere inside living bodies or objects with movements inside.
    we will need scanners of 0.1 nm of resolutions for living patients.

  7. one of the worst speaker. slow words, inadequate aggressive intonations, lack of facial expressions. almost impossible to perceive such presentation, if you are not a masochist, of course.

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