Kurt Thorn (UCSF): Confocal Microscopy
GET UPDATED VERSION OF THIS TALK AT: https://www.ibiology.org/talks/confocal-microscopy-short-course/ Confocal microscopy is a powerful technique for acquiring three-dimensional images of biological samples. Here I discuss the basic principles of confocal microscopy, with specific discussions of the operation of laser scanning and spinning disk confocal microscopes and of their application to biology.
Thanks for great explanation
Thanks, Thanks ! So great video.
Awesome!
LIKE.
yes… Awesome!
Excelente! Muchas gracias, esto me ayudó mucho. 🙂
Thanks for an excellent, easy to follow introduction to confocal microscopy! That would have taken me hours to read:) Keep up the great work.
Awesome!
like
Fantastic. Very well explained.
awesome!
Excellent work Kurt!!! I've addad a link on my blog –
Really nice video, thanks for uploading this. I suggest this to anyone who is going to use a confocal as its going to make your life a lot easier and will make it all a lot clearer. Thanks
very interesting thanks
Confocal Microscopy starts at 3:50
Awesome!
Very nice video! thanks for uploading 🙂
explained very clear.
Extremely thorough and well-prepared discussion of a topic in which I had no previous understanding. Totally great!
Very nice presentation there. However, if it was in HD (720p or better), I would have seen and maybe understood more 😉
Thank you for making this video.
Excellent presentation!
is there a difference between the dichromatic mirror and dichrioic mirror?
very clear… helped me a lot… thanks..:)
wait.. working of spiral spinning disc is not very clear..
spinning spiral mirror working is not very clear
Good explanation of the diagrams. Thank you!
Thank you. Very precise and informative.
Great video.
very helpful, thank you 🙂
Excellent!
Thank you!
Very helpful, thanks!
Thanks a lot
a life saver !
Great help. Thanks!
That's just extra ordinary talent. To make such a complicated thing so simple
Why is this potato quality?
Nice one!
If only this video was in higher resolution…
good presentation…Checkout my live cell 3D timelapse confocal videos of yeast cells immobilised on a chamber slide..those working with live yeast cell imaging will appreciate the ability to add drugs etc without loosing the focud
I am stuck, can't get it. trying hard to understand how to see molecular interactions using fluorescence and confocal microscope. is there any possibility to do a video before this and explain basics of how light interacts with specimen?
Thank you so much for this excellent presentation!!! ❤
Thank you so much for this excellent presentation!!! ❤
Thank you so much!!
Thank you so much!!
Confocal and multi-photon way to go! Spent years in research using, and later selling LOL!
fantastic!!
Great! Thanks
Excellent video. Very easy to follow. Thank you!
awesome vid, thanks to iBiology and Nikon! Wish the resolution was higher though.
Crazy to think that after a few years (from the date of this video in 2010) laser scanning confocal has gotten so efficient with the new generation of insanely fast galvano-resonance hybrid scanners that spinning disk is getting phased out since you can get pretty much very close frame rates at comparable resolutions (something like 30 fps, 512×512) without the small compromise in optical sectioning that you get in spinning disk (which is not as bad as some people make it out to be though). Although 2-photon's still the boss, if you've got the $$$$$$ hehe
Excellent presentation, very didactic, thanks Kurt
Very helpful, thanks!
excellent video..i have a question please,i want to do a fluorescent exerience with confical and 1 EMCCD camera ..what is the difference between using 2 camera or 2 …thank you
"you can see that there is stuff that is clear and sharp and in focus.." yeah.. nah not really :'D
Thank You very much
need elctron microscope for hiv diagnoses
If only microscopists and histology people added scale bars. Don't show me an image without context!
This sounds inspired by early innovations in the history of television.
13:45 Apparatus.
18:28 Spinning disk.
22:30 Multi-photon.
hell yes
this is such a good one! Thank you so much
Thanks for this wonderful video
Thank you for the very helpful video. I have one question.1. Why do we limit to 100µm imaging depth by confocal microscopy? Does imaging depth get better by decreasing pinhole size?
Thank you is very much for the video!
Thank you for the amazing explanation