Kurt Thorn (UCSF): Confocal Microscopy

GET UPDATED VERSION OF THIS TALK AT: https://www.ibiology.org/talks/confocal-microscopy-short-course/

Confocal microscopy is a powerful technique for acquiring three-dimensional images of biological samples. Here I discuss the basic principles of confocal microscopy, with specific discussions of the operation of laser scanning and spinning disk confocal microscopes and of their application to biology.

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  1. Thanks for an excellent, easy to follow introduction to confocal microscopy! That would have taken me hours to read:) Keep up the great work.

  2. Really nice video, thanks for uploading this. I suggest this to anyone who is going to use a confocal as its going to make your life a lot easier and will make it all a lot clearer. Thanks

  3. good presentation…Checkout my live cell 3D timelapse confocal videos of yeast cells immobilised on a chamber slide..those working with live yeast cell imaging will appreciate the ability to add drugs etc without loosing the focud

  4. I am stuck, can't get it. trying hard to understand how to see molecular interactions using fluorescence and confocal microscope. is there any possibility to do a video before this and explain basics of how light interacts with specimen?

  5. awesome vid, thanks to iBiology and Nikon! Wish the resolution was higher though.
    Crazy to think that after a few years (from the date of this video in 2010) laser scanning confocal has gotten so efficient with the new generation of insanely fast galvano-resonance hybrid scanners that spinning disk is getting phased out since you can get pretty much very close frame rates at comparable resolutions (something like 30 fps, 512×512) without the small compromise in optical sectioning that you get in spinning disk (which is not as bad as some people make it out to be though). Although 2-photon's still the boss, if you've got the $$$$$$ hehe

  6. excellent video..i have a question please,i want to do a fluorescent exerience with confical and 1 EMCCD camera ..what is the difference between using 2 camera or 2 …thank you

  7. Thank you for the very helpful video. I have one question.1. Why do we limit to 100µm imaging depth by confocal microscopy? Does imaging depth get better by decreasing pinhole size?

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