Illumina Sequencing by Synthesis
Explore the Illumina workflow, including sequencing by synthesis (SBS) technology, in 3-dimensional detail. Go from sample preparation, to cluster generation, to sequencing on a system flow cell with the proprietary SBS process, through to data analysis on the BaseSpace® Sequence Hub. Want more details? Download an introduction to Illumina next-generation sequencing technology for an in-depth […]
what does it mean 'the number of cycles determines the length of the read?' at 2:45 .. I thought all fragments are read at the same time.. Please someone help 😣
this is one of the best videos for sequencing by synthesis. thanks a lot
I have a question. It seems during the first read all the two types of oligos have been occupied through bridging amplification. Why there are free oligos to be used for bridging during the second read?
Hi @illumina do you allow the showing of your videos in lectures on NGS for educational purposes?
Hello, thank you for the nice video. It is indeed very helpful.
One question that has been addressed before by another person (@quickfastgoninja) in the comments:
Considering that both oligo complements ('purple' and 'blue') are present in the flow cell:
what is preventing the "blue" sequences from hybridizing when the DNA is flowed across the cell? From the animation, it seems that purple:purple hybridization would be equally as likely as simultaneous blue:blue hybridization. Are the "blue" adapters somehow blocked from hybridization when the samples are first flowed across the cell?
This is so helpful! I was trying to understand what really was going on during the process, which the lecture slides from my lecturer didn't seem to help much. Thanks!
I still do not get it why after sequencing the first strand and before proceeding to adding index 2 primer to generate clusters of the other strand index 1 is added again (3:17) to generate a short read. Reading the comments below has not helped. Is anyone able to explain this again? Is this done to signal to the data acquisition computer: "this is the end of sequencing data for the fragments labelled with index 1"?
thanks
Why do the nucleotides have to bind sequentially?
Wtf is the purpose of the music in these videos?
woah this is actually so smart
You go straight into it with the unexplained technical jargon don't you
Thanks 🤗
Click the following link to get a better understanding. Old video by Illumina. Simple but better animation (especially index reads). Same explanation.
https://www.youtube.com/watch?v=HMyCqWhwB8E
what
I am now understanding how DNA sequencing works clearly!
Only one word: AMAZING
high quality video, exciting to watch
Thank you so much, it's so great and clear video
Thanks for the upload!
Hi, I have a few questions that need urgent answers:
1/ How flourescent label in the blocking group are removed from the nucleotide?
2/ How to wash away free nucleotide?
3/ Why do we have to sequece 2 strain of DNA?
4/ What is the function of index 1 and 2 in adapter attached to DNA?
Thank you.
I do that at work everyday
Where can I hire use of this machine in the UK?
It is bad and very bad
The second and third phase is that a primer?
I mean, oligos = adaptor = primer right?
This explains better than me prof. on class
I think the cleaving off of the fluorescent signal and removal of terminator during sequencing by synthesis is a key step that is omitted in the video. It becomes very confusing, then, how the previously added nucleotide's flourescent does not interfere with the newly added nucleotide.
anyone know what end is the 3', purple or blue in the video?
This some dank <3
Thank you for this 😀
Need some more explanations on index 1 and index 2
what happen if we dont have the reference gene to align back the contig seqs?
you tell a lie
and scamming me
can someone link me the music?
It is clear for me, thanks
I've go to study this for my genetic engineering exam
Did anyone else get the chills at (2:01) or was that just me?
I have one question… your DNA is sequenced by ilumina miseq is of 5MB size. U have to get 10x coverage . How much data do u need?
Yeah,, I dont understand the concept enough to invest in it. Theranos feel!!
Please drop the music
Not helpful at all. This video raises more question than answers to my initial questions.
stfu
when could I exert my effort to a company like this?🤯 I'll do my best in college first.✊
does anybody know if the fluorescent molecule is attached to the nucleotide and before the next nucleotide is added does it has to be removed in order to get a monochromatic signal?
Mubeena
I wonder what is the physical proximity of the oligos to be able to bend.
Seq chain of cod to brok liga en g ob to mde co 19
How is the read length determined? At 4:10
How the Oligo fold over ?
Amazing video with clear explanation but i have small doubt, here i found that when the reads are cleaved and washed off their respective indices are not ligated to their respective reads, how will this work in demultiplexing??
0:11c
Next Generation Sequencing is really amazing!
Hi everybody. Here is a Sars-CoV-2 question.
In his presentation paper of "how to make a RT-PCR test for COVID" (https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2020.25.3.2000045#html_fulltext), the german Christian Drosten explains he sequenced the virus without any virus isolates or original patient specimens. I understand he used all the available sequences (full sequences?, part of sequences ?) of all the Cov-like viruses already known and registred in Databank, and then "aligned them". What the hell does that mean ? Did he "create" ONE sequence "gathering" all the Cov-like sequences ? A sort of "Virtual virus" ?
And then, as far as i understand as well, he choosed three different parts of his "created alignement" supposed to be caracteristic of the "new Coronavirus" and confirmed it with the first publication of the genome by the Chines on january 11th ?
His test method is the one recommanded by WHO and used in this pandemic from the start. As scientific and reliable is it ? Just asking.
I passed my college watching Youtube videos
The loud, repetitive dinging in the background is unfortunately quite distracting.
I wish there was no music. It's too loud and the pitch is so high because of the harmonics that you can't omit it. At least if you have anything at all to do with music
bruh what????
helloo, why do we do the step before bridging ( the replication and detachment of one strand..) thanks !
Amazing…
biology is soooo easy pfffffffffffffffffffffffffff
physics is way cooler
This could be applied to cosmetic without surgery if applied concurrent with Active MRI and Lighting treatment and coating optic gel
What is index?
I wanted the speedrunner illumina not this crap
Data analysis is the real D and A
0:43 what's indices?
Why a read 2 (4:00) is nessesary? How come just read 1 (2:23) is not sufficient?
Wait the name is IlluminaHD‘s!
very helpful..
Complimenti, argomento molto interessante! 😊 Se siete interessati alla scienza vi consiglio anche il mio canale: https://www.youtube.com/channel/UCCyLPYz_mGOaadbHe_H2i4Q
So question about experience, in terms of jobs asking to do things such as experience in PCR, NGS library prep, etc., is experience actually necessary? from being a bit exposed to it and reading it, it just seems as if you just need to follow protocols and instructions in order to do the procedures? Or can anyone elaborate on this?
Aveces no me gusta mi carrera y otras veo este tipo de cosas y me enamoró más de mi carrera y de la ciencia jajaja ❤️
Нихуя не понял конечно, но я старался
What does Reduced Cycle Amplification means?
I contracted herpes' I was told there is no Herpes cure except treatment to control it. I totally lost hope. All I could think was losing my life because it was so embarrassing to have this virus, few weeks ago I read about a possible natural cure which was guaranteed. And I ordered the treatment after some weeks I got 100% cure. Now I'm so excited to share this testimony thanks Dr Abumere: http://www.drabumereherbalremedy.home.blog
Can anyone help me
Before the first sequencing, how the reverse strand are cleaved and washed off , leaving only forward strand?
I heard this is a minecraft speedrunning channel, guess I got trolled
Hey guys, we have a gift for you
Ive pressed the replay button 6 times
Thank you so much! Visualizing the process while reading about this technique is too difficult, an animated illustration is the perfect solution! ❤️
Shut up science bitch
Tf
Shyful islambhuyan
Wait a minute…this isn’t a Minecraft speed run
Mire like Illuminati
How come no polymerase is needed to add the building nucleotides to the cluster?
Si vous vous êtes là à cause de Chatron manifestez-vous
who even comes up with something like this, gotta have 10000000 IQ
This is already becoming outdated lmao. Nanopore and PacBio ftw!
Don't know if they meant to show these details, but the letter ordering in the forward and reverse passes is reversed but not complemented- is this an error? in 2:35 you see the ordering (from top to bottom) of AATTCGCATCG and at 3:56 on the reverse read the order is reversed but not complemented i.e. GCTACGCTTAA- shouldn't it have been complemented i.e. (C<>G,A<>T)?
This is also used for whole genome sequencing correct?
The best lecture vedio
tldr just use ion torrent sequencing so you dont receive brain damage
Can anyone help me to understand what is an NGS assay? Thank you very much in advance.
This could have been explained so much better really
I am wondering what the background music in this video is?
sheeeeesh
oeoe promo pass 2021 2022
What is the purpose of the indices in the adaptors mentioned at 0:40?